FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8213743
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTT129900.021.5226151
CTTGAAA102850.021.4127312
ACGAAGT221550.021.406093
GACGGCT161450.021.3171061
GCGCGAT135650.021.307861
TACACGA134700.021.2457621
AGGGATG125800.021.2357481
CAAGAGA124950.021.2310454
CACAAGA123950.021.1844962
TGACTAG101550.021.1711371
CCTACCA103000.021.1401521
ACTTGTG175600.021.1364882
GTATTCG151250.021.13552
GTAGCCT174700.021.13378
CAACTAG107950.021.1288661
CACGAAG224350.021.128212
TCACAAG168000.021.1278171
ACAAGAG125000.021.1257653
TTGACGA77850.021.09202410
CGCGATT96550.021.0895062