FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8217751
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA137000.021.44031
GGAACTT135850.021.4274431
GCGCGAT133800.021.410411
GACGGCT151650.021.393041
ACGAAGT225650.021.363933
CCTACCA105050.021.3217011
TGACTAG100600.021.3025251
CTTGAAA104300.021.2716392
CACAAGA124700.021.2407262
GTAGCCT179550.021.231098
CAAGAGA125150.021.2241974
CACGAAG227550.021.1920532
AGGGATG125100.021.1581881
GTAACGT145800.021.1497731
GCGATTC96700.021.1445243
GTGTCAA144900.021.1390728
CTACGTC137500.021.1303081
GGGATGC122200.021.1261942
TCACAAG166150.021.1217771
CTTGTGT184550.021.1036153
Produced by FastQC (version 0.11.5)