FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13613244
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT368000.021.6024971
CAACTAG290150.021.352211
AGCTTGA168500.021.3263931
TTACTCG343800.021.3255732
GAGAGTT259450.021.2532844
CTCCTAA245650.021.2413232
CTCCCAT147900.021.2400027
CCACCTA234450.021.2329581
ATTACTC349150.021.198951
TGAGAGT319650.021.1863753
CTTGAGT163450.021.1574213
CTAGGGA231300.021.1285636
AGATTGC162500.021.0572431
TCGTTAG229400.021.0398436
GGCCGAA171800.021.0318139
AGCTCAT223600.021.0057474
ACTAACC154600.020.9752642
CGGAGCT229100.020.9697511
AGGCCGA172050.020.9692868
AGCCTAG233450.020.9687163
Produced by FastQC (version 0.11.5)