FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37272
Sequences flagged as poor quality0
Sequence length8
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAAT643217.25692208628461No Hit
ACATTCCG442711.877548830221077No Hit
TGTGATGC439111.780961579738142No Hit
CTGCGGTA416611.17729126421979No Hit
GACACTAT397010.651427344923803No Hit
GACACGAT28757.713565142734493No Hit
AACACAAT19495.2291264219789655No Hit
CACACAAT15794.236424125348788No Hit
GACAGAAT7161.9210130929383988No Hit
GACAAAAT7071.8968662803176648No Hit
TACACAAT6951.8646705301566862No Hit
GACACAAA5811.5588109036273878No Hit
GATACAAT5081.3629534234814338No Hit
CGGCGGTA4751.2744151105387422No Hit
ACATTCCA3200.8585533376261001No Hit
GACACAAC2410.6465979823996566No Hit
ATGCGGTA2120.5687915861772913No Hit
ACTTTCCG1700.45610646061386567No Hit
ACACTCCG1650.44269156471345783No Hit
GTCACAAT1610.4319596479931315No Hit
GACACACT1460.3917149602919081No Hit
GACGCAAT1170.3139085640695428No Hit
ACATTGCG1130.30317664734921657No Hit
GGTGATGC1120.30049366816913503No Hit
CTGCCGTA1000.2682979180081563No Hit
ACAGTCCG1000.2682979180081563Illumina DpnII expression Adapter 1 (100% over 8bp)
TGTGATGA990.2656149388280747No Hit
GAAACAAT890.23878514702725906No Hit
CCGCGGTA880.2361021678471775No Hit
CCATTCCG880.2361021678471775No Hit
CTGCGTTA880.2361021678471775No Hit
GACATAAT870.23341918866709596No Hit
GACACCAT830.22268727194676968No Hit
GTGCGGTA750.2012234385061172No Hit
CTGCAGTA730.19585748014595405No Hit
GGCACAAT720.19317450096587252No Hit
CTGGGGTA700.18780854260570937No Hit
TGTCATGC620.1663447091650569No Hit
TTGCGGTA540.14488087572440436No Hit
CTGCGATA530.14219789654432283No Hit
TTTGATGC520.13951491736424126No Hit
ACATTCAG460.12341704228375189No Hit
TGTGATGT430.11536810474350719No Hit
TGCGATGC420.11268512556342562No Hit
GCCACAAT390.10463618802318093No Hit
ACATTTCG380.10195320884309937No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)