FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150074
Sequences flagged as poor quality0
Sequence length8
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACTA5157234.36438023908205No Hit
TACAACTA3800025.320841718085745No Hit
GTTGGTAC94586.302224236043552No Hit
AGGCTGGT65194.343857030531605No Hit
CACAAATA64264.281887602116289No Hit
CACAACTA61074.069325799272359No Hit
CACAACTT53693.5775683995895355No Hit
GTTGGTCC50523.366339272625505No Hit
TCATCAAG48113.205751829097645No Hit
GTGGGTCC20941.3953116462545145No Hit
TCATCAAT17131.1414368911337074No Hit
GTTTGTCC15571.0374881725015659No Hit
GGTGGTCC7920.5277396484401029No Hit
CGGCTGGT6660.4437810680064502No Hit
GTTGGACC6010.4004691019097245No Hit
AGGCTGGA5700.37981262577128616No Hit
AACAACTA4920.32783826645521547No Hit
GTAGGTCC4500.2998520729773312No Hit
GACAACTA4280.28519263829843944No Hit
CAAAACTA3970.2645361621600011No Hit
CACAACAA3890.25920545864040406No Hit
CACAAGTA3840.25587376894065594No Hit
CACATCTA3760.2505430654210589No Hit
GGGCTGGT3300.2198915201833762No Hit
AGGGTGGT3270.21789250636352733No Hit
GTTGGTGC3240.21589349254367846No Hit
TAATCAAG3120.20789743726428297No Hit
CACGACTA3020.2012340578647867No Hit
CGCAACTA2920.19457067846529047No Hit
GTTGGGCC2730.1819102576062476No Hit
TGGCTGGT2580.1719151885070032No Hit
CACTACTA2210.14726068472886708No Hit
CACAACTG2140.14259631914921972No Hit
GTTGGTCA1990.13260125004997536No Hit
AAGCTGGT1980.13193491211002573No Hit
CACAACCA1940.12926956035022721No Hit
CACAATTA1890.1259378706504791No Hit
GTCGGTCC1760.11727547743113396No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)