FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23360
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCGCT723930.988869863013697No Hit
CATTGATA475020.333904109589042No Hit
ATGGTTAG450419.28082191780822No Hit
GCAAACGT13615.826198630136986No Hit
TGCCGGCT9704.152397260273973No Hit
GCAAACGC7833.3518835616438354No Hit
GCAAACAC5872.5128424657534247No Hit
GCAAACGA4321.8493150684931507No Hit
CATTGATC4111.759417808219178No Hit
ATGGTTAA3051.3056506849315068No Hit
GTGGTTAG2641.13013698630137No Hit
TGCCCGAT1520.6506849315068494No Hit
CAATGATA1270.5436643835616438No Hit
CATTGAAA1030.4409246575342466No Hit
CATTCATA860.3681506849315069No Hit
CATTGAGA810.3467465753424658No Hit
CTTTGATA790.3381849315068493No Hit
GGAAACGC750.3210616438356164No Hit
CATTAATA710.3039383561643836No Hit
AATTGATA630.2696917808219178No Hit
GGCCCGCT530.2268835616438356No Hit
GCAAACCC490.20976027397260275No Hit
GCAATCGC450.19263698630136986No Hit
GCAAACTC380.16267123287671234No Hit
TATTGATA370.1583904109589041No Hit
CCTTGATA350.14982876712328766No Hit
TGCCCGCA340.14554794520547945No Hit
TGCCTGCT330.14126712328767124No Hit
CATTGACA310.1327054794520548No Hit
GTAAACGC300.12842465753424656No Hit
ATGGGTAG290.12414383561643837No Hit
CCAAACGC250.10702054794520548No Hit
CAGTGATA250.10702054794520548No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)