FastQCFastQC Report
Fri 10 May 2024
EGAF00005395171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74138
Total Bases593.1 kbp
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCTA2961939.95117213844453No Hit
ATAACATA840411.335617362216407No Hit
CGGTGAGC53357.196039817637379No Hit
TCCGAGCG45776.173622164072405No Hit
ATAACCTA43375.8499015349753165No Hit
GATCTTAT41455.590925031697645No Hit
AAAACCTA21502.899997302328091No Hit
TATCTTAT18972.558741805821576No Hit
CTAACCTA11451.5444171679840297No Hit
ATAACTTA11361.532277644392889No Hit
ATAAACTA11181.5079985972106071No Hit
ATAACCCA8111.0939059591572473No Hit
ATAATCTA7350.9913944266098358No Hit
GGGTGAGC6980.9414874962907012No Hit
AGAACCTA6250.8430224716070032No Hit
CGGTGATC6150.8295341120612911No Hit
GATCTGAT5980.8066039008335807Illumina PCR Primer Index 9 (100% over 8bp)
ATAACCAA5320.7175807278318811No Hit
ATTACCTA5240.7067900401953114No Hit
GATCTAAT5080.6852086649221721No Hit
CGGTGACC4590.6191157031481831No Hit
CGGTGAAC4080.5503250694650517No Hit
TCCGAGAG3690.49772046723677466No Hit
GTAACCTA3610.48692977960020506No Hit
ATATCCTA2940.39655777064393427No Hit
CGGGGAGC2220.2994415819148075No Hit
GATCTCAT2080.28055787855081066No Hit
CGGTGGGC2080.28055787855081066No Hit
ATAACCGA2070.27920904259623947No Hit
AATCTTAT1670.22525560441339124No Hit
GCCGAGCG1640.22120909654967763No Hit
TCCGATCG1390.1874881976853975No Hit
ATAAGCTA1360.1834416898216839Illumina PCR Primer Index 10 (100% over 8bp)
ATAACGTA1240.16725565836682943No Hit
TTAACCTA750.10116269659284037No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)