FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28025
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAAG639622.822479928635147No Hit
CCGAACTC587720.970561998215878No Hit
AACGGTCA543919.407671721677076No Hit
TTATTGGT448416.0No Hit
AAAGGTCA10723.825156110615522No Hit
CCCAACTC6042.1552185548617304No Hit
GGTCAAAG4341.5486173059768065No Hit
GGTCCAAT4141.4772524531668152No Hit
CCGAACAC3361.1989295272078502No Hit
AACAGTCA2270.8099910793933988No Hit
CCGATCTC2240.7992863514719001Illumina Paired End PCR Primer 2 (100% over 8bp)
AACGCTCA2210.7885816235504014No Hit
AACGGTAA2060.7350579839429081No Hit
CCAAACTC1900.6779661016949152No Hit
GGTCCAGG1700.6066012488849242No Hit
GCGAACTC1380.49241748438893845No Hit
CCTAACTC1340.47814451382694023No Hit
GGTCCAAA1270.45316681534344333No Hit
AACGATCA800.2854594112399643No Hit
AACCGTCA730.2604817127564674No Hit
GGCCCAAG710.25334522747546834No Hit
GGGCCAAG680.24264049955396969No Hit
GGTCCATG490.17484388938447815No Hit
GGTCCACG460.1641391614629795No Hit
GGTCCCAG420.14986619090098127No Hit
AACGGTTA420.14986619090098127No Hit
TTATTGAT410.1462979482604817No Hit
AACTGTCA410.1462979482604817No Hit
GGTACAAG400.14272970561998216No Hit
GTATTGGT390.13916146297948262No Hit
GGTTCAAG380.13559322033898305No Hit
CACGGTCA350.12488849241748438No Hit
ATCGGTCA340.12132024977698484No Hit
CGGAACTC340.12132024977698484No Hit
GACGGTCA290.10347903657448707No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)