FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149871706
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGGG3874335525.851013532867906No Hit
TCACTCCT3862092725.76932499854242No Hit
CGTGCTAA3718304224.80991442107158No Hit
ATCTGATC3366639722.46347752924091Illumina PCR Primer Index 9 (100% over 8bp)
GAGAAGGA3194320.21313696128874385No Hit
TCACTCCA2444320.1630941600144326No Hit
ATCTGATA1824060.12170809612322689No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)