FastQCFastQC Report
Fri 10 May 2024
EGAF00005395195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109455572
Total Bases875.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGACT3481398531.80649862210761No Hit
TCCTATGA2409742622.01571428451354No Hit
ATACTGAG2023393018.485975295985845No Hit
GATGCCTC1990680818.187112484323777No Hit
ATACGGAG91339278.344871652582473No Hit
GATGCCTA1828430.16704768579529236Illumina PCR Primer Index 3 (100% over 8bp)
CGGAGACA1825600.16678913340291163No Hit
TCCTATAA1771410.16183826621453315No Hit
ATCCTGAG1153540.10538887869500146No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)