FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106094042
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATCAA2889362227.233972290357265No Hit
TTCCATCT2756637225.98295953320357RNA PCR Primer, Index 14 (100% over 8bp)
ACTGGAGC2742516325.849861578466392No Hit
CGGTCGTG2122218720.003184533208753No Hit
CGGTCGTA1775920.16739111513915172No Hit
TTCCATCA1519120.14318617439422282No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)