FastQCFastQC Report
Thu 9 May 2024
EGAF00005395253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24909521
Total Bases199.2 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTCG813938932.675815002624894No Hit
CGTGCAGA617286124.78113087762707No Hit
GTATGCTC531282521.32849122229207No Hit
AACAAGAT484022219.43121266763821No Hit
AACCAGAT716790.28775744021733696No Hit
AACAGGAT456860.18340778210869652No Hit
CGTTCAGA427260.1715247756068854Illumina RNA PCR Primer (100% over 8bp)
TCGCTTCA336610.13513306819508894No Hit
GTATGCTA263120.10563029293096404No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)