FastQCFastQC Report
Wed 1 May 2024
EGAF00005395258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178980319
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGACTA4726226526.406403376675176No Hit
AACACAGC4481090825.036779602566245No Hit
CGGTTTAG4215566023.553237716600563No Hit
GTACGGCT4184862223.381689245955585No Hit
CGGTGTAG5721310.3196614036652823No Hit
CGGGTTAG5641360.31519443207607645No Hit
CGGTTTAA2595590.14502097294842792No Hit
GTACGGCA1903600.10635806275437469No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)