FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12288714
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCCTT361064429.381788851136093No Hit
TCACATCA296720124.14574055511423Illumina PCR Primer Index 1 (100% over 8bp)
AGTGGAAC282645723.000429499783298No Hit
CAGATGGG272455922.17122963395519No Hit
GTCTCCTA230170.1873019422536809No Hit
CAGATGGA229030.18637426178198954No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)