FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135441565
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCGAA4026114129.725838593197No Hit
GCGCACCT3854028828.455288448564513No Hit
TGTAGATC3116920723.01302927207021Illumina Single End PCR Primer 1 (100% over 8bp)
CTATTTGG2397913017.704410016230984No Hit
CTATTTGA3483340.25718397450590597No Hit
GCGCACCA1950650.1440215195387029No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)