FastQCFastQC Report
Tue 7 May 2024
EGAF00005395294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28792821
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT322786911.210672966014688No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT7489082.601023359260282No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG4745001.6479802378516504No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC3559351.236193563666443No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT2486790.8636840412406968No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC2148070.7460436058002097No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA2147990.7460158210965157No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC1468990.5101931484935082No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT1373260.4769452774356497No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA1195380.41516598877199284No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG1083180.3761979418411277No Hit
GCGGTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT731740.2541397385132912No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC686440.2384066500465515No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC625880.21737362935017726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG555210.192829316724471No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA540580.18774818903642682No Hit
GGGACGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT485610.1686566245106723No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC469770.16315525317925603No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA463210.16087690747634628No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT428680.14888433474441423No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT410740.1426536149410299No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTCTT398240.13831225498883906No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATCGAAGTTATT383230.13309914995824826No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC372360.1293239033438231No Hit
GCGTTATCCACCTTCCACTGTACGTTGGCCTCTCTGGGATAGAAGTTATT345780.12009243554148447No Hit
GCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA332640.11552879795974143No Hit
CTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCAC319920.111111030072392No Hit
GCGTTATCCACCTTCCACTGTACTGTGGCCTCTCTGGGATAGAAGTTATT299640.10406760768595755No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGCTAGAAGTTATT289180.10043475767796423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)