FastQCFastQC Report
Sun 5 May 2024
EGAF00005395344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373053298
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCAT12519095233.55846273740756No Hit
CCGGATCC9765408626.176979676507244ABI Dynabead EcoP Oligo (100% over 8bp)
AACTTAGA8733707223.411419351665938No Hit
TTAACGTG5880611515.763462034853799No Hit
CCGGATCA9879680.26483293548044173No Hit
AACTTAAA4993220.13384736247526755No Hit
TTAACGTA4633030.12419217374135103No Hit
GGTCGCAA3877020.1039267048645687No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)