FastQCFastQC Report
Wed 8 May 2024
EGAF00005395353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15790980
Total Bases126.3 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGACGT464816229.435551181750597No Hit
ATCCCACA410675926.006992599572666No Hit
GGAATGTC358088822.676793967188864No Hit
CCTTGTAG325626720.621057084487475No Hit
CCTTGTAA290320.18385179387219794Illumina PCR Primer Index 12 (100% over 8bp)
ATCCCAAA203560.1289090354113551No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)