FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141939614
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGG3607396725.415009935140446Illumina Paired End Adapter 2 (100% over 8bp)
CTTGTTAT3555347525.048310332871555No Hit
GGCCCATC3510128524.72973119399916No Hit
TCATAGCA3364093723.70087958672341No Hit
GGCCCATA2769620.19512664026266832No Hit
TCATATCA2227140.1569075705672977No Hit
AAGAGCGA2005880.14131925143885485No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)