FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118268537
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGCGT3442604629.108372246119863No Hit
CTCCAACA2997614825.345834792900163No Hit
ACAACTTG2786643823.56200449152423No Hit
TGTGTGAC2503796021.17043182837376Illumina PCR Primer Index 5 (100% over 8bp)
ACAACTTA1767260.14942773833416068No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)