FastQCFastQC Report
Wed 1 May 2024
EGAF00005395405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19597469
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT4928882.5150594701795423No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3303411.6856309353008798Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1442880.7362583402989437No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT862680.44019970129816255No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG745330.3803195198318722No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT595030.3036259427173989No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC584890.29845180517953623No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG502690.2565076133045548No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT428000.2183955489354263No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT403240.20576126437551706Clontech SMART CDS Primer II A (100% over 24bp)
GCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTC321250.16392422919510677No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA312420.15941854532337824No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA254410.1298177841230416No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT219430.11196854042733782Clontech SMART CDS Primer II A (100% over 25bp)
GTCCCAGGTCACCATCACGCCCTGGTGCCTGGGGCGCCCCACGATGGAGG218080.11127967596223777No Hit
GGGAAGTTTCTGGCGGTCAAAGTAGTAAGACATGGACAGGTAAACGTAGG211150.10774350504139081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)