FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25117270
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAGAA701506727.92925743920418No Hit
GTAATTGC550323821.910175747603144No Hit
TCGTCACG549250321.8674362301317No Hit
AGTCGCGT496357019.761582369421518No Hit
AGTCGCTT18985047.558560305319806No Hit
TCGTCACA443670.17663941980955733No Hit
AGTCGCTG371720.14799379072646032No Hit
AGTCGCTA263840.1050432630616305No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)