FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139609344
Sequences flagged as poor quality0
Sequence length8
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGT4787298534.29067398239476Illumina Paired End PCR Primer 2 (100% over 8bp)
GTCATATA4557064432.641542961479715No Hit
CATCGTCG3749865926.859705751500417No Hit
TCGGACAC75320465.395087308769247No Hit
CATCGTCA2229060.15966409812798776No Hit
GTCATAAA1738520.12452748148433389No Hit
AGATCGGA1586440.11363422780641388Illumina Single End Adapter 2 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)