FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005395441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37911466
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT441205211.637777341556774No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT6269801.6538004623719904No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA5355711.4126887100593788No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT3203940.8451110806424631No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG1540500.4063414482573689No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA1381290.36434623762636875No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG1129240.29786239339834547No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1091790.2879841154124718Clontech SMART CDS Primer II A (100% over 26bp)
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT962110.253778105019732No Hit
GTCACAGACTGAAAGTCTGGAGACTGAGTCAGCACAATTTCACCCCTGGA750980.19808782915437773No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT739060.1949436616352425No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG714850.18855773079310625No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG694480.183184686131631No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG617290.16282409126568728No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC606430.15995952253600534No Hit
GCTGGTACCAGTGTAAGCTACTACCAATGCTCTGACTGGCCCGGCAGGTG588710.15528547484816335No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG543910.14346846940711816No Hit
GGCCCGGCAGGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGAAAGT531610.14022406835968831No Hit
GGTTTCTGCTGGTACCAGTGTAAGCTACTACCAATGCTCTGACTGGCCCG511430.1349011404623604No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT500220.13194425137767027No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC467280.1232555871091875No Hit
GTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCTGAGATGGACTG465110.12268320090813686No Hit
GGGAAGCATACTTGATGAGGAGCTTTGGAGACTGATCTGGTTTCTGCTGG446950.11789309334542747No Hit
GATCTGGTTTCTGCTGGTACCAGTGTAAGCTACTACCAATGCTCTGACTG429370.11325597379958875No Hit
GTAAGCTACTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTTTC428880.11312672530257734No Hit
GGTACCAGTGTAAGCTACTACCAATGCTCTGACTGGCCCGGCAGGTGATG427740.1128260247176936No Hit
GCTTTGGAGACTGATCTGGTTTCTGCTGGTACCAGTGTAAGCTACTACCA427050.11264402173210607No Hit
CACAGACTGAAAGTCTGGAGACTGAGTCAGCACAATTTCACCCCTGGAGG422230.11137263855742219No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA421590.11120382419397869No Hit
GGAGCTTTGGAGACTGATCTGGTTTCTGCTGGTACCAGTGTAAGCTACTA414160.10924399494337676No Hit
GAAAGTCTGGAGACTGAGTCAGCACAATTTCACCCCTGGAGGCTGGAACC412090.10869798598661419No Hit
CTTTCTCCTTTGGAGTCACAGACTGAAAGTCTGGAGACTGAGTCAGCACA408050.10763234531737707No Hit
GTCTCAGGGACCCCCCAGGCTGAACTAAGCCTCCCCCGGACTCCACCAGC389180.10265495932022255No Hit
GGTGACTTTCTCCTTTGGAGTCACAGACTGAAAGTCTGGAGACTGAGTCA387770.10228304017576106No Hit
ACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTTTCTCCTTTGGAG385950.1018029743297186No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)