FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005395446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31909032
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT331741410.396473324543345No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT5821751.8244834252571496No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA2316570.725991938583408No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT2042730.640172976729598No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG1696060.5315297562144787No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1588340.4977712893327507No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA1305130.40901585482129327No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC996330.31224074738462765No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC730450.22891637703080434No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG690750.2164747586200672No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC585830.1835937862358219No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG565250.1771442016793239No Hit
CCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGC466350.14614984246466642No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA458750.14376807168578476No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC445090.13948715210163692No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAGCTGGTGCTAATAGTCTGACTGGC438440.13740310267011546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG399570.1252215986997036No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT398210.12479538708664055No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG396700.12432216683978381No Hit
GATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTG362920.11373582250943871No Hit
GCTCCTTCCCCGGGAGACAAAGACAGGGTGTCTGGAGACTGCGTCAAGAC348270.10914464594225233No Hit
GCTGGTACCAGGCTAAGTAGCTGGTGCTAATAGTCTGACTGGCCCTACAG340410.10668139353146157No Hit
GTCTGACTGGCCCTACAGGAGAGGGTGGCTCTTTCACCTGGAGACAAAGA339030.10624891410055937No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG327190.10253836593977529No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323880.10150104208739394Clontech SMART CDS Primer II A (100% over 26bp)
GTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGAT322840.1011751155597575No Hit
GGAGCCTGGGAGCCTGGTCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTG322630.10110930347244629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)