FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42645272
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGA1390536332.60704492634025No Hit
CCAAGATG999466623.43675050308039No Hit
TACGTGAC906871021.265452357766648RNA PCR Primer, Index 17 (100% over 8bp)
GTTTATCT872185520.45210310770207No Hit
GGTTATCT1415420.3319054923603254No Hit
GTGTATCT578280.13560237111396545No Hit
CCAAGTTG569880.13363263341361734No Hit
GTTTATCA524540.123000739683405No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)