FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005395471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56208144
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGCGC1956815934.813743360748575No Hit
GAGGATCT1376689224.492699847908163No Hit
AGACCATA1047420818.634680412148104No Hit
CTTATGAG971350217.2813071358485No Hit
CTTAGGAG12153402.1622133618217316No Hit
CTTCTGAG1753470.3119601316136679No Hit
GTGGATCT1352090.2405505508240941RNA PCR Primer, Index 23 (100% over 8bp)
CTGATGAG966540.17195728789763987No Hit
CTTATGAT758300.13490927578039225No Hit
AGATCATA738060.13130837410322604No Hit
TCCTGCGA701260.12476128014474201No Hit
GAGGATCA665470.11839387544979246No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)