FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14050539
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT376000.021.542521
AGCTTGA172750.021.4085271
CCACCTA240900.021.360451
ATTACTC365600.021.3424531
CAACTAG302500.021.3115671
CTCCTAA245950.021.3061282
TTACTCG362800.021.289722
ATGTAAT237950.021.1949146
CTCCCAT147700.021.1869757
GAGAGTT264000.021.1867084
TCGTTAG242650.021.1470286
CTAGGGA236000.021.1463186
TGAGAGT323800.021.144473
CTTGAGT169350.021.1400593
GGCCGAA178500.021.1236159
AGCCTAG237200.021.1042793
AGCTCAT225550.021.0823424
GCTCCTA253900.021.0037421
ACTCGAC223500.020.9995869
GAGCAGT194450.020.997934
Produced by FastQC (version 0.11.5)