FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12038807
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT336700.021.5949171
CAACTAG262500.021.4095541
AGCTTGA145600.021.3405511
CTCCTAA207600.021.3350472
CCACCTA202300.021.2951681
ATTACTC318600.021.2222231
ATGTAAT208500.021.2126756
TTACTCG315500.021.1990492
AGCCTAG210550.021.1792933
GAGAGTT224700.021.16694
CTTGAGT142750.021.157113
CTAGGGA210200.021.1405416
GCCTAGG209800.021.1392564
CTCCCAT128950.021.1370227
GACTAAC140600.021.097431
TGAGAGT279000.021.0939773
AAGAAGT95950.021.04706610
TAGCCTA211950.021.0217762
GGCCGAA152950.021.0133159
TGTAATA210850.021.0125947
Produced by FastQC (version 0.11.5)