FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11082634
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT301550.021.6466921
CAACTAG239650.021.3916871
CTCCCAT114300.021.3346587
ATTACTC285600.021.3295861
AGCTTGA137050.021.2969551
TTACTCG282950.021.2721042
CTCCTAA201000.021.2461592
CCACCTA186500.021.2268031
CTAGGGA189950.021.2114186
CTTGAGT133750.021.2066943
GAGAGTT210250.021.2043274
TCGTTAG185450.021.192316
TGAGAGT260450.021.1386263
GAGCAGT155500.021.1153284
AGCCTAG190350.021.0971493
ATGTAAT190550.021.0926766
GGCCGAA147150.021.0792459
GCCTAGG190500.021.0583654
TAGCCTA190400.021.0477562
ATAGTAG122800.021.00442110