FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11410137
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT319700.021.5622231
CAACTAG245250.021.3624821
CCACCTA199950.021.3581711
TTACTCG296050.021.3092352
CTCCTAA199150.021.24552
ATTACTC300800.021.2448141
GGCCGAA144450.021.1765619
GAGAGTT213800.021.1757954
AGCTTGA138700.021.1641251
CTCCCAT124000.021.1562527
AGCCTAG203200.021.1490543
AGGCCGA145000.021.141758
CTTGAGT134600.021.1380963
CTAGGGA203400.021.1175736
TAGCCTA203400.021.1153622
TGAGAGT264900.021.0908033
GAGCAGT162150.021.069614
ATGTAAT194500.021.0433046
ACCTAGT158450.021.0324293
TTAAGAA85250.021.0183539