FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9864934
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC169050.021.6165121
AGACGAC207050.021.4580888
CAGACGA205800.021.4073437
TCTGCCA242300.021.387652
GACGACG207800.021.364659
AAACCGG224600.021.3344522
ACCCACT176050.021.3318561
CCTACCA167150.021.3291651
TCAGACG207100.021.3208756
CAGGTAA290600.021.3191782
TAAACCG226450.021.2620621
CCTTCTC193000.021.259595
ATCTGCC249350.021.2548791
TACAATG192850.021.2478226
CAAACTT119500.021.2365072
ACGACGT208700.021.23562210
CCCACTC171100.021.2290442
CAATGGA200550.021.2266128
GGAAACG172200.021.20777510
CTCGGAA173100.021.2062917
Produced by FastQC (version 0.11.5)