FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7489011
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC131600.021.531981
TCTGCCA187300.021.3422992
AGACGAC152750.021.3228578
CCTACCA126400.021.3212661
CAGGTAA220100.021.3203742
TCAGACG151950.021.3195766
ACCCACT142150.021.3190941
CAGACGA151200.021.316067
TACAATG146700.021.2952256
CAATGGA151600.021.2886988
GACGACG152950.021.27349
CAGCATA150500.021.2545491
CGGAAAC134100.021.2533239
CCCACTC138400.021.2529542
TAAACCG172250.021.246511
AAACCGG171200.021.2418862
CAAACTT92100.021.2237342
GGCTGGT159600.021.2074581
CTCGGAA134450.021.1899577
ATCTGCC192200.021.1873681
Produced by FastQC (version 0.11.5)