FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7489011
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT119345915.936136293563996No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1451791.9385603786668224No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1091241.4571216412954928No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA945471.262476447157041No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC808481.0795550974621348No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC797651.0650939089286957No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA566710.7567220825286544No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT561400.7496316936909293No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC391900.5233000725997065No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG390920.5219914885957572No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT260950.34844387329648735No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG229960.30706324239609206No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT228330.3048867200221765No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG209340.27952956672115986No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC167550.22372780598132383No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG147550.19702200998235947No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG144110.1924286130705376No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT143490.19160073339456973No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC115770.15458650014000513No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA115620.1543862066700129No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG114080.15232986037809265No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG112570.15031357278017085No Hit
GCGGTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT106540.1422617752864831No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA103560.13828261168263742No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91660.12239266306325362No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGC88950.11877402770539396No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAGAGAAGTTATT88530.11821320598941569No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG87480.11681115169947007No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC87150.11637050606548714No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC81180.10839882595979629No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC79560.10623565648388018No Hit
GCGTTATCCACCTTCCACTGTACGTTGGCCTCTCTGGGATAGAAGTTATT75600.10094790887608523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1396350.084.7357941
CGTTATC1413150.083.5811542
GCGGTAT21800.080.9385151
TTATCCA1481850.079.7124
CGGTATC22050.079.603832
TATCCAC1540300.077.807395
ATCCACC1554950.077.377726
GTTATCC1537250.076.974783
CACCTTC1614800.074.25429
CACGCTG130700.073.583531
CGTAATC4100.072.561572
TCCACCT1659400.072.299777
CCACCTT1671750.071.7249768
CGTGATC6250.067.320552
CGTTAAC4350.063.5062642
CGTTAGC2550.063.3338552
CGTTATA4300.063.2563322
ACGCTGC154150.062.3926622
GCGTTAA4850.062.2415281
GCGTTAG2600.062.140371
Produced by FastQC (version 0.11.5)