FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7114562
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC123350.021.6259961
CCTACCA120350.021.4978351
AGACGAC145250.021.4390668
CAGACGA144550.021.4215857
CAGGTAA204050.021.4183242
TAAACCG166450.021.405761
TCTGCCA182400.021.3612772
GACGACG145900.021.3360149
ATCTGCC186100.021.3207871
AAACCGG166150.021.3186152
TACAATG136850.021.3012266
TCAGACG146100.021.2927976
ACCCACT129200.021.2257611
CAAACTT83350.021.2218842
CAATGGA143700.021.218698
CCCACTC125900.021.2054542
TAAGGTA205950.021.1940216
CTCGGAA128250.021.1936957
CGGAAAC127950.021.182769
ACTCGGA124850.021.1811436
Produced by FastQC (version 0.11.5)