FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7107102
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC127000.021.6541041
CCTACCA123500.021.4572351
CAGGTAA206200.021.440422
ACCCACT131050.021.3962041
TAAACCG166800.021.3608671
GGAAACG128950.021.3513410
CGGAAAC129350.021.3193289
CAATGGA143650.021.3104788
ATCTGCC184750.021.3098891
TACAATG138450.021.3091776
AGACGAC144950.021.2938928
AAACCGG165800.021.2906592
GACGACG145050.021.2564629
TAAGGTA207800.021.2487166
TGAAAGA157600.021.2467441
CCCACTC127800.021.2431182
ACTCGGA127000.021.2295346
CAGACGA144200.021.2221667
TCTGCCA183050.021.2133332
ACGACGT145400.021.21285810
Produced by FastQC (version 0.11.5)