FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7192981
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC128100.021.6394881
TCTGCCA176650.021.4521662
AGACGAC147650.021.3889398
CAGGTAA205050.021.3832192
CAGACGA146550.021.3619867
TCAGACG147450.021.358576
TACAATG140250.021.3491616
CCTACCA121250.021.3469471
TGAAAGA157750.021.3446751
TAAACCG163650.021.3346961
GACGACG147900.021.3230369
TAAGGTA205950.021.311146
CAATGGA146850.021.2807438
GTCAGAC148000.021.2568975
ACTCGGA128350.021.2459556
GGCTGGT149500.021.2422811
ACAATGG141750.021.23957
CTCGGAA130950.021.2355797
AAACCGG163850.021.2280942
CGGAAAC130750.021.225859