FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7190028
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC126400.021.5737531
CCTACCA119150.021.4277881
TCTGCCA178850.021.3914362
GACGACG148950.021.3718559
CAGGTAA209650.021.360212
TCAGACG148850.021.3426156
AAACCGG161100.021.3380032
AGACGAC149300.021.3366368
TAAACCG162550.021.323461
CAGACGA148800.021.2980387
ATCTGCC183400.021.2924331
CAAACTT86150.021.2852922
ACCCACT134250.021.279131
ACGACGT149350.021.27042410
TAAGGTA210050.021.2619256
CAATGGA147050.021.2442078
ACTCGGA126950.021.2378186
AGGTAAG211050.021.223733
TCAAGAC157550.021.2183487
GACTTCT88050.021.2007772
Produced by FastQC (version 0.11.5)