FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7950959
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGA67650.020.9268424
GAATGTG97250.020.8585132
CCTACCA86800.020.8477041
CCCAGCT87650.020.80764210
TTGAGAC74900.020.78092210
CGTTGCT100900.020.75706710
TAGTGGT103300.020.7550411
GAGGTGA75900.020.7400551
AATGTGT97750.020.7284033
TGAGCCC62200.020.72676
CGAATGT100250.020.7061611
AGATCCC89250.020.6811148
GCGACCA74150.020.6509531
CTTAATC101100.020.6180810
CGCCAGA54750.020.613715
CTACACC93400.020.5876431
ATAACGC78400.020.5838241
TGAGCCG73700.020.5830061
TCCTTAA101350.020.5780758
AGAGTGA52500.020.5541678
Produced by FastQC (version 0.11.5)