FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8857866
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGTG108250.020.92422
CCGTAGA77500.020.8078964
ATCTTGA83750.020.778317
AATGTGT109050.020.7494683
GAACCTA57550.020.739931
CCTACCA94400.020.7196621
TAGTGGT116150.020.7039781
CGTTGCT108600.020.70330210
AGATACA77950.020.7015348
ATAACGC85200.020.6845381
CGAATGT111850.020.6737651
AAACCTG86850.020.6588921
CTTGAGA84400.020.6443529
AGATCCC99000.020.6220268
GGACCCT62000.020.61593410
GCGACCA81750.020.6155011
TTGAGAC84500.020.60690510
TAGATAC77950.020.6027557
CCCAGCT93350.020.59755510
ATTAGGA85650.020.5617435
Produced by FastQC (version 0.11.5)