FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10632234
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCT128400.020.8689310
CCCAGCT110550.020.84554310
TTGAGAC99200.020.83545910
CCTACCA111450.020.797211
GCGACCA99900.020.7901271
CGAATGT128500.020.7087251
ATCTTGA100400.020.7069457
GAATGTG126500.020.7054842
GCCGTAG91550.020.6904983
CCGTAGA90300.020.6723674
GAATGAA56400.020.6555841
AGTGGTT137150.020.6216222
GGAGCGA69900.020.614949
TAGTGGT139950.020.6101151
CTACACC125550.020.6082551
TGAGCCG92900.020.6041241
TAGAGAG74550.020.5832797
ATAACGC108500.020.5819681
CTTAATC136300.020.57937810
ACTCCAT144350.020.5675354