FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12503921
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCTA238800.021.4702281
GTGACCC320150.021.41866510
AGTTAGA239400.021.4111947
TTAGAAC243150.021.3659789
TGTGACC321200.021.365779
CTACCCA325350.021.362161
GCCTCCA306850.021.3297425
TACCCAG322650.021.32952
AGACTAG262850.021.321388
AGAATAG205600.021.3036561
TAGAACA244300.021.29241610
TCAACCA390900.021.2845738
GTGTGAC325900.021.2702778
TGCCTAA234350.021.2300342
CCCAGTG322200.021.2148724
CGTCCAT308050.021.1974961
ATTCAAC300150.021.1791786
CCTCCAA308300.021.16526
CATAGTT244150.021.162094
ATTAGGA273000.021.1579745