FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12408377
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCTA236950.021.4683761
AGTTAGA242150.021.3677647
TCAACCA381300.021.3616358
GGGTGAA131900.021.331332
CTACCCA331450.021.3231751
TTAGAAC246250.021.3068079
AGACTAG256950.021.3056728
TACCCAG327450.021.3047392
GCCTCCA301900.021.2963245
AGAATAG202550.021.2736721
GTGACCC325450.021.26236510
TGCCTAA232400.021.2589682
TAGAACA247300.021.24302910
GTGTGAC329450.021.2379268
TGTGACC326300.021.2272059
ATTCAAC298450.021.2181666
CGTCCAT307400.021.2088181
CATAGTT246150.021.195154
CCTTAGA159100.021.1906134
CCTCCAA302950.021.175316