FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5163250
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACCC131700.021.39808710
GCCTCCA125600.021.3863965
CTGCCTA96950.021.3808061
CTACCCA135000.021.377431
AGTTAGA95700.021.3672717
TGTGACC131950.021.3658819
TACCCAG133500.021.329172
GTGTGAC133850.021.3173488
TTAGAAC97600.021.3119569
TCAACCA165000.021.2994568
CCTCCAA125650.021.2640826
CCTTAGA66400.021.237414
TAGAACA98100.021.2369710
CGTCCAT128800.021.2361741
ATTAGGA108300.021.227535
AGACTAG106100.021.221898
CCGGGAT82400.021.2171781
CCAGTGT132700.021.2119675
GTAAGCT118450.021.1915255
TAGTTAG96050.021.1748896
Produced by FastQC (version 0.11.5)