FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117513
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCTA100100.021.6221561
AGTTAGA99450.021.424017
TTAGAAC101800.021.3940669
GCCTCCA125950.021.3792955
TGTGACC126350.021.3462269
TAGAACA102150.021.3315310
GTGACCC126400.021.3290810
GTGTGAC127850.021.2936638
CCTTAGA64800.021.2694474
GGGTGAA55050.021.2673322
TCAACCA157300.021.2649148
CCTCCAA126300.021.2416676
CTCCAAC126050.021.2399567
TGCCTAA97500.021.239472
CTACCCA129100.021.2312771
CATAGTT101000.021.226124
AGACTAG106200.021.2119888
TCCAACC127850.021.2076288
TACCCAG127600.021.1962932
CCAGTGT127300.021.1871345
Produced by FastQC (version 0.11.5)