FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12922951
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG40000.018.5936641
CCTACCA41550.018.5090621
ATGCGAT39100.018.233771
GACTAAC38750.018.1997151
GGAACTT31700.018.0130121
GGCTCGA32900.017.9913861
CGGACGT52250.017.9403761
TCCCGAT44900.017.9367181
TGCCAAA41250.017.8968581
CGCTGGA35350.017.896031
CAATGGA42950.017.7739438
GGGACCT33300.017.7422331
GATCTAG37300.017.6683621
ACACCCT44550.017.6577991
CTAGCCT47900.017.5024031
CCCTCCT55150.017.3760431
CTGAAAC31800.017.36821
AAACCTG35150.017.3405441
CTCGAAA30250.017.3125021
AAATGCC33150.017.2583031
Produced by FastQC (version 0.11.5)