FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13156438
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG36350.018.6478141
CGGACGT55050.018.6098981
GACTAAC40050.018.546111
GGCTCGA32750.018.5136431
CCTACCA44950.018.4338861
CGGACTG37750.018.3351881
GTAACTG45000.018.0221521
GGGACCT34900.017.9406621
GGAACTT36400.017.8966581
ATGCGAT39450.017.8797991
GATCTAG38900.017.821431
CTGAAAC30650.017.8074951
CTTAACT53200.017.7884641
CGCTGGA39700.017.6840531
CCCTCCT56450.017.6415311
CAATGGA43350.017.6346058
ATGAATG27900.017.62273610
ACTGAAC27650.017.5507261
TCCCGAT44350.017.5419311
TGCCAAA40650.017.5144021
Produced by FastQC (version 0.11.5)