FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12528751
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCTA239950.021.465191
AGTTAGA237100.021.4563057
GTGACCC319550.021.36916710
AGACTAG264900.021.359638
TGTGACC320850.021.3237239
TCAACCA386650.021.3134868
TGCCTAA234200.021.3015632
TTAGAAC243750.021.2680449
GCCTCCA301750.021.2564775
TAGAACA244000.021.24625410
CTACCCA326650.021.2416461
CCCAGTG322550.021.2089444
CCTCCAA301100.021.207386
TACCCAG323300.021.2064952
TTCAACC297750.021.2048327
GTGTGAC325950.021.1858088
CATAGTT242250.021.173724
GTAAGCT281250.021.1709845
CGTCCAT305950.021.1683331
CCTTAGA161450.021.162114