FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18771433
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC58350.018.42241
CCTACCA62000.018.3671231
CGGACTG53650.018.190571
CGGACGT76350.018.1574121
TGACTAG51300.018.0158351
GGCTCGA45150.018.0085661
ATGCGAT54800.017.9493771
GGAACTT47700.017.7839581
GGGAATG46000.017.651881
CTGAAAC46550.017.5851351
GATCTAG56250.017.5844841
CCCTCCT79150.017.570681
TGCCAAA57050.017.569331
GGGACCT49250.017.5593621
TGCTACC61650.017.5255181
TCCCGAT61000.017.38761
CGCTGGA53500.017.3778231
AGAAACC46850.017.303858
AGCTTGA42200.017.2859611
TCTTGAG36100.017.2766978