FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12434403
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCTA235900.021.4650671
TGTGACC330300.021.393139
AGTTAGA237450.021.3923827
GTGACCC330700.021.37057910
GTGTGAC334100.021.3111298
AGACTAG255900.021.307188
TCAACCA385600.021.2974858
CTACCCA337800.021.263471
TACCCAG335350.021.2416382
ATTCAAC294300.021.2409296
GCCTCCA305900.021.2229525
CGTCCAT302300.021.2199341
CCCAGTG332550.021.2124274
TTAGAAC243850.021.1872799
AGAATAG206000.021.1782251
TAGAACA244250.021.15708410
CCAGTGT334100.021.1535245
TTCAACC295150.021.1271537
GGGTGAA131750.021.120872
TGCCTAA231200.021.1114142
Produced by FastQC (version 0.11.5)